Plant Tissue Culture:

Plant Tissue Culture is a fascinating and useful tool which allows the rapid production of many genetically identical plants using relatively small amounts of space, supplies and time. Basically the technique consists of taking a piece of a plant (such as a stem tip, node, meristem, embryo, or even a seed) and placing it in a sterile, nutrient medium where it multiplies.
In recent times there are huge demands of Plant tissue culture techniques in various fields like-

• Micropropagation is widely used in forestry and in floriculture. It can also be used to conserve rare or endangered plant species.
• A plant breeder may use tissue culture to screen cells rather than plants for advantageous characters, e.g. herbicide resistance/tolerance.
• Large-scale growth of plant cells in liquid culture inside bioreactors as a source of secondary products, like recombinant proteins used as biopharmaceuticals
• To cross distantly related species by protoplast fusion and regeneration of the novel hybrid
• To cross-pollinate distantly related species and then tissue culture the resulting embryo which would otherwise normally die (Embryo Rescue).
• For production of doubled monoploid plants from haploid cultures to achieve homozygous lines more rapidly in breeding programmes, usually by treatment with colchicine which causes doubling of the chromosome number.
• As a tissue for transformation, followed by either short-term testing of genetic constructs or regeneration of transgenic plants.
• Certain techniques such as meristem tip culture may be employed that can be used to produce clean plant material from virused stock, such as potatoes and many species of soft fruit.

Basic course in Plant Cell culture Technology: (Two weeks)

• Basic requirement of Plant tissue culture laboratory
• Different methods of sterilization of glassware, plastic wares, culture media.
• Preparation of M.S. media and demonstration of callus culture.
• Maintenance of culture and subculture scheduling.
• Micro propagation of Sugarcane using apical meristem culture.
• Preparation of rooting media, and demonstration of hardening of the rooted plants.
• Application

Advance course in Plant Cell Culture Technology: (Three-Four weeks)

• Basic requirement of Plant tissue culture laboratory
• Different methods of sterilization of glassware, plastic wares, culture media.
• Preparation of M.S. media and demonstration of callus culture.
• Maintenance of culture and subculture scheduling.
• Micro propagation of Sugarcane using apical meristem culture.
• Somaclonal variation and characterization of somaclones using RAPD (DEMO)
• Somatic embryogenesis in carrot.
• Preparation of synthetic seeds and germination of synseed.
• Protoplast isolation and viability test.
• Preparation of rooting media, and demonstration of hardening of the rooted plants.
• Green House Demonstration and visit to green house.
• Application.

Batch Size :   10 Students
Eligibility   :   BSc, MSc, Lecturers, Research students, scientists and Biotechnologists & entrepreneurs